供應expedeone標記試劑盒Metal Labeling kits批發(fā)  欣博盛供

目前的金屬偶聯(lián)操作步驟復雜且耗時，并可能導致抗體回收率較低。

Expedeon的新產(chǎn)品Lightning-Link Metal Labeling Kits耗時短（可以在30秒內建立金屬/抗體標記），操作簡單（只需將抗體溶液添加到激活的金屬配體即可）。

操作步驟

特點和優(yōu)勢

• 手工操作只需30s
• 1h即可完成標記
• 一次只需10μ或100μg的標記物標記您的抗體
• 無分離步驟，十分抗體回收率
• 溫和的化學反應，穩(wěn)定的偶聯(lián)物質
• 良好的批間重復性
• 應用范圍廣

規(guī)格

·         1x 10 μg

·         1x 100 μg

10種供選擇的金屬元素:

139La, 141Pr, 144Nd, 151Eu, 153Eu, 154Sm, 159Tb, 165Ho, 169Tm, 175Lu

應用

• X射線熒光
• 質譜流式細胞術
• 多重離子束成像
• 解離放大鑭系熒光免疫分析

應用舉例

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link® 159Tb at 100 μg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link® metal labels at 10 μg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue? (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link® Metal Labeling kit.